p53-mediated DNA renaturation can mimic strand exchange
نویسندگان
چکیده
منابع مشابه
MutS inhibits RecA-mediated strand exchange with platinated DNA substrates.
Human cell lines and Escherichia coli dam mutants are sensitive to the cytotoxic action of the anticancer agent, cisplatin. Introduction of mutations disabling DNA mismatch repair into these cell lines renders them resistant to the action of this drug. We used RecA-mediated strand exchange between homologous phiX174 molecules, one that was platinated and the other that was unmodified, to show t...
متن کاملCasein kinase 2 inhibits the renaturation of complementary DNA strands mediated by p53 protein.
Considerable effort is currently being devoted to understand the functions of protein p53, a major regulator of cell proliferation. The protein p53 has been reported to catalyse the annealing of complementary DNA or RNA strands. We report that this activity is inhibited in the presence of the serine/threonine protein kinase CK2. It is shown that this inhibition can be explained by the occurrenc...
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Rad51, Rad52, and RPA play central roles in homologous DNA recombination. Rad51 mediates DNA strand exchange, a key reaction in DNA recombination. Rad52 has two distinct activities: to recruit Rad51 onto single-strand (ss)DNA that is complexed with the ssDNA-binding protein, RPA, and to anneal complementary ssDNA complexed with RPA. Here, we report that Rad52 promotes annealing of the ssDNA str...
متن کاملDNA Strand Exchange Promoted by RecA K72R
Replacement of lysine 72 in RecA protein with arginine produces a mutant protein that binds but does not hydrolyze ATP. The protein nevertheless promotes DNA strand exchange (Rehrauer, W. M., and Kowalczykowski, S. C. (1993) J. Biol. Chem. 268, 1292–1297). With RecA K72R protein, the formation of the hybrid DNA product of strand exchange is greatly affected by the concentration of Mg in ways th...
متن کاملHomologous pairing and DNA strand-exchange proteins.
PRINCIPLES OF HOMOLOGOUS PAIRING AND DNA STRAND EXCHANGE . Homologous Pairing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ... . . . . . . . DNA Strand Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Energetic Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Experimental Assays . . . . . . . . . . . . . . . . . ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1997
ISSN: 1362-4962
DOI: 10.1093/nar/25.20.4004